Detailed view for TcCLB.506559.190

Basic information

TDR Targets ID: 54557
Trypanosoma cruzi, kinatase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.3518 | Length (AA): 1285 | MW (Da): 143061 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00782   Dual specificity phosphatase, catalytic domain
PF13855   Leucine rich repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0004725   protein tyrosine phosphatase activity  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0016791   phosphoric monoester hydrolase activity  
GO:0008138   protein tyrosine/serine/threonine phosphatase activity  
GO:0016311   dephosphorylation  
GO:0006470   protein amino acid dephosphorylation  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 30 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
104 404 1vzo (A) 46 321 25.00 0.0000000026 1 0.42 -0.17
107 416 1fot (A) 87 357 28.00 0.0000000015 1 0.36 0.26
123 411 1xbb (A) 369 639 23.00 0 1 0.47 -0.53
189 406 2bfx (A) 143 349 25.00 0.0000047 1 0.5 -1
438 738 1wmk (A) 2 299 16.00 0 1 0.48 -0.64
440 731 1uu3 (A) 73 359 16.00 0 1 0.44 -1.03
889 1134 1h6t (A) 32 287 22.00 0 1 0.34 0.38
921 1063 1dce (A) 418 565 30.00 0.000000000094 1 0.52 -1.18
1128 1278 2esb (A) 21 170 29.00 0 1 0.65 -1.6
1128 1265 1zzw (A) 323 462 36.00 0 1 0.72 -1.85
100 407 4e1z (A) 885 1160 18.00 0 1 0.334289 0.13
105 335 1b6c (B) 188 412 32.00 0.000000087 1 0.335167 0.23
107 416 1fot (A) 87 357 28.00 0.000000001 1 0.356645 0.33
127 415 2rku (A) 47 323 26.00 0.00000027 1 0.379303 0.04
129 394 4xg8 (C) 373 622 26.00 0.0000008 1 0.429404 -0.34
130 416 4rz7 (A) 537 807 24.00 0.0000022 1 0.384746 -0.03
175 446 3q5i (A) 87 373 25.00 0.00000000018 1 0.456073 0.04
179 416 5k3y (A) 133 353 23.00 0 1 0.412014 -0.73
203 402 2bva (A) 367 574 28.00 0.0000087 1 0.372042 -0.08
234 335 3soc (A) 294 398 37.00 0.0000022 0.46 0.287777 0.76
302 400 5cek (A) 247 338 31.00 0.42 0.9 0.424443 -0.69
322 398 3aln (C) 288 365 41.00 0.18 0.92 0.128322 -0.08
465 715 3ddq (C) 17 288 25.00 0.0000085 1 0.405731 0.08
509 717 1phk (A) 83 291 17.00 0 0.78 0.327246 -0.09
900 1160 5a5c (A) 34 276 34.00 0 1 0.370513 0.61
941 1044 4nkh (A) 278 370 44.00 0.000026 0.93 0.422334 0.6
970 1032 2z7x (B) 29 90 39.00 0 0.92 0.436827 -0.21
1122 1279 1yz4 (A) 0 153 27.00 0 1 0.543557 -0.93
1127 1269 2hcm (A) 11 153 30.00 0 1 0.607084 -1.62
1200 1270 1r6h (A) 96 160 31.00 0.04 0.2 0.195853 0.77

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_138464)

Species Accession Gene Product
Dictyostelium discoideum DDB_G0278445   leucine-rich repeat-containing protein
Dictyostelium discoideum DDB_G0269918   leucine-rich repeat-containing protein
Dictyostelium discoideum DDB_G0270688   leucine-rich repeat-containing protein
Entamoeba histolytica EHI_099820   leucine rich repeat and phosphatase domain containing protein
Leishmania braziliensis LbrM.20.1690   dual specificity protein phosphatase, putative
Leishmania donovani LdBPK_341950.1   kinatase, putative
Leishmania infantum LinJ.34.1950   dual specificity protein phosphatase, putative
Leishmania major LmjF.34.2190   dual specificity protein phosphatase, putative
Leishmania mexicana LmxM.33.2190   dual specificity protein phosphatase, putative
Trypanosoma brucei gambiense Tbg972.4.2420   dual specificity protein phosphatase, putative
Trypanosoma brucei Tb927.4.2460   kinatase, putative
Trypanosoma brucei Tb11.v5.0751   dual specificity protein phosphatase, putative
Trypanosoma congolense TcIL3000_4_2340   kinatase, putative
Trypanosoma cruzi TcCLB.510949.10   kinatase, putative
Trypanosoma cruzi TcCLB.506559.190   kinatase, putative

Essentiality

TcCLB.506559.190 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.2460 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.2460 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.2460 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.2460 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 24.9% 245 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 23.0% 369 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 21.7% 345 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 26.8% 153 aa Compounds References
Patiria pectinifera Cdc2 300 aa 23.0% 256 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 26.2% 252 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0088 0.4477 0.5
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0032 0.5 0.5
0.0069 0.3067 1
0.0039 0.9485 0.5
0.0067 0.5 0.5
0.0042 0.5 0.5
0.0093 0.8828 0
0.0036 0.5 0.5
0.0092 1 0.5
0.0081 1 0.5
0.0012 0.5 0.5
0.0007 0.5 0.5
0.0039 0.5 0.5
0.0059 1 1
0.0033 0.5 0.5
0.0059 1 1
0.0026 0.5 0.5
0.0091 1 0.5
0.0066 0.3101 0
0.0023 0.5 0.5
0.0056 1 0.5
0.0037 1 0.5
0.0029 0.5 0.5
0.0007 0.5 0.5
0.0061 0.6883 0.5304
0.0033 1 0.5
0.0027 1 0.5
0.0016 0.5 0.5
0.0003 0.5 0.5
0.0008 0.5 0.5
0.0011 1 0.5
0.0098 0.3242 0.3649
0.0012 0.5 0.5
0.0007 0.5 0.5
0.0032 0.5 0.5
0.0062 0.6935 0
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0063 1 0.5
0.0022 0.5 0.5
0.0063 0.7244 0.2543
0.0039 0.5 0.5
0.0059 1 1
0.0064 0.3377 0
0.0018 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

17 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.506559.190 (Trypanosoma cruzi), kinatase, putative
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